Back to Program | 2010 Program and Abstracts Overview | 2010 Posters
Transoperatorial Lavage with An Electrolyzed Solution By Ionic Selectivity Considerably Decreases the Bacterial Load and the Inflammatory Response in a Murine Model of Sepsis
Francisco Nachon Garcia*1,2, Jose Diaz Tellez2, Luis Landero Lopez3, Ma. Gabriela Nachon Garcia4, Fabio Garcia-Garcia4, Juan Santiago Garcia5, Armando J. Martinez6
1Instituto de Ciencias de La Salud, Programa de Doctorado en Ciencias Biomedicas, Universidad Veracruzana, Xalapa, Mexico; 2Cirugía General, Centro de Especialidades Medicas del Estado de Veracruz, Xalapa, Mexico; 3Patología, Centro de Especialidades Medicas del Estado de Veracruz, Xalapa, Mexico; 4Depertameneto de Biomedicina, Instituto de Ciencias de la Salud, Universidad Veracruzana, Xalapa, Mexico; 5Instituto de Investigaciones Biologicas, Universidad Veracruzana, Xalapa, Mexico; 6Instituto de Neuroetologia, Universidad Veracruzana, Xalapa, Mexico
Introduction Peritoneal sepsis is the prototype of a severe and potentially fatal disease. To treat this problem, hemodynamic and metabolic support, antimicrobial treatment, and control of the source of infection are required. Transoperative peritoneal lavage (TOPL) with 0.9% saline solution (SS) has been the standard method to eliminate the bacterial load and biological detritus from the abdominal cavity. The addition of antibiotics or antiseptics to the SS used for TOPL has proved ineffective and toxic. In this study we tested Electrolyzed Solution by Ionic Selectivity (ESIS-0205, ProdInnv Mexico), as an alternative for TOPL, whose mechanism as antibacterial is attributed to its high redox potential. We have demonstrated that this solution causes no damage to peritoneal mesothelial cells. Materials and Methods We conducted a randomized double-blind experimental study on a murine model of peritoneal sepsis by cecal ligation and puncture. The care and use of animals was according with APS guiding principles. We compared the effectiveness of SS and ESIS for TOPL by evaluating the systemic inflammatory response (SIR), including cardiac rate (CR), respiratory rate (RR), core temperature (T), white blood cells count, plasma levels of IL-6 and IL-10, histopathological changes in the peritoneum, antibacterial activity, IL-6 and IL-10 mRNA expression in peritoneal mesothelial cells, and animals’ survival rate. Results Clinical parameters of SIR showed significant differences in ESIS group compared to SS group, CR 270 ± 24 vs 299 ± 15, RR 90 ± 1.8 vs 87 ± 3.9, T 32.9 ± 0.7 vs 32.7 ± 0.7 , WBC 5.2 with 2.3% band cell vs. 5.6 whit 4.9% band cell (p <0.001). Plasma levels of IL-10 were higher in ESIS group, 1.5 fold above the control, and 0.5 above to that on sepsis stage (p <0.001). Peritoneal inflammation was reduced in the ESIS group compared to the sepsis stage and SS group (p <0.01, p <0.05 respectively). Bacterial load decreased from more than 350,000 CFU in the sepsis stage, to less than 50,000 CFU (p <0.001) after ESIS treatment. Compared to the sepsis stage or SS treated animals, IL-6 and IL-10 mRNA expression decreased after ESIS treatment (p <0.001). Finally, the Kaplan-Meier analysis showed 50% higher cumulative survival rate in the ESIS group compared to the SS group (p < 0.05). Conclusion This study demonstrates that TOPL with ESIS cause a significant reduction of the bacterial load; resulting in a better control of the local and systemic inflammatory response, which allows longer survival of the animals.
Back to Program | 2010 Program and Abstracts Overview | 2010 Posters
