Introduction: For the better understanding of the pathophysiological events occurring in the sequence inflamation-metaplasia-carcinoma in esophageal adenocarcinoma established rat model have been used in the past. In order to study theses events on a molecular level mouse models would be desirable. This has not been successful in the past due to the high mortality involved.
Methods: Thirty BALB-C mice weighing between 22 - 25g underwent a esophago-jejunostomy as previously described in the rat model. After euthanisation between weeks 24 and 30 the esophgogastric junction and the esophagus were harvested and analysed histopathologically.
Results: Overall mortality was high with 33%, but these fatal outcomes happened during the first 10 animals. After that only 2 animals died due to the procedure. In 18 out of the 20 analysed animals a carcinoma was found just above the anastomosis, histologically an adenocarcinoma. All animals showed a severe esophagitis indicating a profound reflux. One animal demonstrated diffuse lung metastasis.
Conclusion: These results demonstrate for the first time the feasibility of a mouse model for esophageal adenocarcinoma. However, a significant learning curve has to be expected when attempting these procedures.