Members Members Residents Job Board
Join Today Renew Your Membership Make A Donation
2006 Abstracts: Hexose Transporter Expression in the Mouse Jejunum: Role of Diurnal Rhythms
Back to 2006 Program and Abstracts
Hexose Transporter Expression in the Mouse Jejunum: Role of Diurnal Rhythms
Javairiah Fatima, Scott G. Houghton, Judith A. Duenes, Michael G. Sarr; Department of Surgery and Gastroenterology Research Unit, Mayo Clinic College of Medicine, Rochester, MN

BACKGROUND: Expression of hexose transporters (mRNA and proteins) is known to have a diurnal variation in rat jejunum, however, the subject remains unexplored in the mouse. Establishing the presence of such diurnal variation in murine small intestine will facilitate study of molecular mechanisms controlling this process. : AIM: To investigate the diurnal variation in the expression of SGLT1, GLUT2, and GLUT5 mRNA and protein levels in mouse small bowel. HYPOTHESIS: Hexose transporter mRNA and protein levels exhibit a diurnal rhythm in the mouse jejunum. METHODS AND MATERIALS: 24 c57bl6 mice (weight 20-25g) were maintained in a strict 12 hour light/dark room (lights on 6AM to 6PM) with free access to food and water. 6 mice were sacrificed at 3AM, 9AM, 3PM and 9PM (n=6 at each time-point). Jejunal mucosa was harvested into appropriate buffers, and snap frozen immediately in liquid nitrogen. mRNA levels were determined by reverse transcription real-time PCR. Protein was isolated and quantified by Western blot technique (semi-quantitative). Transporter mRNA and protein levels were expressed relative to the housekeeping gene glyceraldehyde-6-phosphate dehydrogenase (GAPDH). RESULTS: mRNA levels of GLUT2 and GLUT5 exhibited a diurnal rhythm with a peak at 3PM (p<0.01). SGLT1 mRNA levels exhibited a diurnal rhythm and peaked at 9PM (p=0.02). Protein levels of SGLT1 and GLUT2 exhibited a diurnal rhythm and peaked 6-12 hours later at 3AM (p≤0.04 each; Fig.). Protein expression of GLUT5 is pending. SUMMARY: mRNA levels of all three hexose transporters showed a daily diurnal variation (anticipatory to darkness). SGLT1 and GLUT2 protein levels peaked 6-12 hours later during the dark cycle when 70% of feeding occurred. CONCLUSION: Hexose transporter mRNA and protein (SGLT1 and GLUT2) levels exhibit a diurnal rhythm in temporal association with the nocturnal eating pattern of mice; protein expression peaks after message expression. These observations will allow study of genetic control of hexose transporter expression.

Fig: SGLT1 expression in the mouse jejunum (similar findings occurred with GLUT2 expression).


Back to 2006 Program and Abstracts


Society for Surgery of the Alimentary Tract

Facebook Twitter YouTube

Email SSAT Email SSAT
500 Cummings Center, Suite 4400, Beverly, MA 01915 500 Cummings Center
Suite 4400
Beverly, MA 01915
+1 978-927-8330 +1 978-927-8330
+1 978-524-0498 +1 978-524-0498
Links
About
Membership
Publications
Newsletters
Annual Meeting
Join SSAT
Job Board
Make a Pledge
Event Calendar
Awards