Members Members Residents Job Board
Join Today Renew Your Membership Make A Donation
2006 Abstracts: Microsatellite DNA alterations of gastro-intestinal stromal tumors are predictive for outcome
Back to 2006 Program and Abstracts
Microsatellite DNA alterations of gastro-intestinal stromal tumors are predictive for outcome
Paulus G. Schurr1, Stefan Wolter1, Jussuf Kaifi1, Uta Reichelt2, Helge Kleinhans1, Robin Wachowiak1, Emre Yekebas1, Tim Strate1, Viacheslav Kalinin1, Hansjoerg Schaefer1, Izbicki Jakob1; 1Department of General, Visceral and Thoracic Surgery, University Medical Center Hamburg, Hamburg, Germany; 2Department of Pathology, University Medical Center Hamburg, Hamburg, Germany

Purpose: In gastro-intestinal stromal tumors (GIST), loss of heterozygosity (LOH) on chromosome 22 has been described. The prognostic value of these and other DNA regions for patient survival remain unclear. Experimental Design: 60 patients who underwent surgery at our institution between 1992 and 2003 were histopathologically re-classified by immunohistochemistry and the GIST consensus group criteria 2001. 23 microsatellite loci on chromosomes 3, 9, 13, 17, 18 and 22 were screened for alterations in tumor and healthy DNA. Survival was calculated by Kaplan-Meier plots. Results: 11/60 patients showed metastases at presentation (18.3%). 13/60 (21.7%) were high-risk GIST. LOH were found in all tumors. 28/60 (46.7%) showed more than 2 LOH in 23 microsatellite marker sites. The frequency of single marker LOH varied from 1.7% to 28.3% among tumors. Frequent LOH were found on chromosome 22 and 17. The correlation of LOH positivity and the consensus scoring was significant (p<0.001, chi-square test). After a median observation time of 33.3 months (95% confidence interval: 23.9-42.6), overall survival was best for very low, low and intermediate risk tumors with only 6/36 death events, whereas 14/24 high risk and metastasized patients had died (p<0.001, log-rank test). Likewise, LOH significantly predicted survival (p=0.014) and the effect was particularly detrimental for LOH on chromosome 17 (p<0.001). Conclusions: LOH are useful phenomena for the prognosis of GIST. Rather than chromosome 22 markers, chromosome 17 markers independently predict survival. From its linkage to chromosome 17, a fundamental role of p53 in the late stages of GIST can be derived.

Back to 2006 Program and Abstracts

Society for Surgery of the Alimentary Tract

Facebook Twitter YouTube

Email SSAT Email SSAT
500 Cummings Center, Suite 4400, Beverly, MA 01915 500 Cummings Center
Suite 4400
Beverly, MA 01915
+1 978-927-8330 +1 978-927-8330
+1 978-524-0498 +1 978-524-0498
Annual Meeting
Job Board
Make a Pledge
Event Calendar