Abstracts
1999 Digestive Disease Week

# 2172 THE BENEFICIAL EFFECT OF IGF-1/BP-3 COMPLEX ADMINISTRATION ON INTESTINAL BACTERIAL TRANSLOCATION IN RAT ACUTE PANCREATITIS MODEL
Hideaki Nomura, S Haji, K Yasuda, H Ohyanagi, Kinki Univ Sch of Medicine, Osaka Japan

[Objective] Bacterial translocation (BT) has been recognized as one of the significant causes complicating systemic infection following major surgery, burn injury and even acute pancreatitis. BT is considered to be closely related with increased intestinal permeability induced by the breakdown of the mucosal integrity. Insulin like growth factor-1 (IGF-1), a peptide growth factor that is regulated by IGF binding protein3 (BP-3), has been reported to be able to reduce BT by improving the mucosal structure and function. In the present study, we investigated whether IGF-1/BP-3 complex, as the new modality of IGF-1 administration, improves the intestinal mucosal structure and function, and reduces BT following acute pancreatitis in rat. [Methods] Acute pancreatitis model was made by injection of 1% deoxycolic acid (DCA) into pancreatic duct in 8-10 week male Wistar rats. IGF-1/BP-3 complex (IGF group) or saline (C group) was injected at dose of 2.6 mg/kg/day subcutaneously for 5 days after DCA injection in each group. [Results] Survival rates for five days were recovered in IGF group compared with in C group (42.5 vs 20%). Wet mucosal weight and protein content of jejunum were significantly higher in IGF group than in C group (0.13±0.6 vs 0.2±0.02g, 11.8±3.3 vs 29.3±6.4 mg/cm, respectively). Intestinal mucosal villus height, which was evaluated by histrogical findings, showed significant higher in IGF group compared with in C group (371.3±76.2 vs 476±65.5 mm). There was a tendency of increased level of jejunal alkaline phosphatase in IGF group (44.5±22.9 vs 67.6±39.9mg/cm). BT, which was estimated by the count of bacterial colony forming in the culture of harvested mesenteric lymph nodes, was reduced in IGF group compared with in C group, resulting the lower level of serum endotoxin (97.5±28.1 vs 33.3±35.1CFU/ml, 535.0±55.7 vs 26.6±34.7pg/ml, respectively). [Conclusion] The IGF/BP-3 complex reduced bacterial translocation by maintaining the intestinal integrity and improved the survival rate in acute pancreatitis model.

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