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1998 Abstract: SMALL BOWEL TRANSPLANTATION (SBT) ALTERS NON-ADRENERGIC NON-CHOLINERGIC (NANC) INHIBITION IN LONGITUDINAL SMOOTH MUSCLE OF THE RAT JEJUNUM. B.M. Balsiger, N. Ohtani, C. Shibata, M.M. Murr, W.J. Anding, J.A. Duenes, M.G. Sarr. Dept. of Surgery, Mayo Clinic, Rochester, MN. 77

Abstracts
1998 Digestive Disease Week

#981

SMALL BOWEL TRANSPLANTATION (SBT) ALTERS NON-ADRENERGIC NON-CHOLINERGIC (NANC) INHIBITION IN LONGITUDINAL SMOOTH MUSCLE OF THE RAT JEJUNUM. B.M. Balsiger, N. Ohtani, C. Shibata, M.M. Murr, W.J. Anding, J.A. Duenes, M.G. Sarr. Dept. of Surgery, Mayo Clinic, Rochester, MN.

AIM: To study effects of SBT on contractile properties of longitudinal smooth muscle of the rat jejunum. METHODS: 8 full thickness longitudinal muscle strips were obtained per Lewis rat 1 and 8 wk after syngeneic orthotopic SBT (SBT1; SBT8). In vitro spontaneous contractile activity was quantitated under baseline and NANC conditions and in response to nitric oxide (NO; 1-30µM). 10-sec electrical field stimulation (EFS) at 1-10 Hz was evaluated in presence or absence of L-NMMA (10-3 M; NO synthase inhibitor), methylene blue (MB; 10-5 M; blocks soluble guanylate cyclase), or tetraethylammonium (TEA; 10-3 M; blocks K+ channels). RESULTS: Spontaneous contractile activity (g·5 min/mg;  ± SEM) increased in SBT1 compared to naive control (NC) (18.2 ± 1.7 vs 9.5 ± 1; p<0.001) and returned to normal in SBT8 (12.3 ± 1.5). NANC conditions decreased spontaneous contractile activity in NC, SBT1 and SBT8 (_% basal activity: -18 ± 2; -17 ± 3; -15 ± 6 resp.); all p<0.03 vs baseline. NO caused similar contractile inhibition in NC, SBT1 and SBT8 (_% baseline activity: -35 ± 13, -45 ± 15,-34 ± 8; all p<0.03 vs baseline). EFS induced a tetrodotoxin-sensitive NANC net inhibition of contractile activity. Maximum net inhibition was shifted to a higher frequency and was augmented in both SBT1 and SBT8 (p<0.05, table); L-NMMA and MB did not alter the response to EFS but TEA abrogated EFS inhibition at all frequencies in all groups. SUMMARY: Spontaneous contractile activity of longitudinal muscle is increased 1 wk but not 8 wk after SBT. SBT caused an increase of EFS-induced net inhibition and a shift of max. inhibition to a higher frequency. CONCLUSIONS: Changes in spontaneous activity and response to EFS indicate that SBT induces an alteration of NANC inhibitory function. NANC neurotransmission is mediated through TEA-sensitive, non-nitrergic neurons in the longitudinal muscle. In the rat jejunum, SBT alters longitudinal muscle function much differently than in circular muscle. (NIH DK39337 [MGS]).

    Frequency (Hz)

1

2

3

4

5

7

10

    NC_

-71 ± 5

-71 ± 4

-74 ± 4

-66 ± 8

-68 ± 6

-45 ± 14

-39 ± 14

    SBT1_

-47 ± 9*

-75 ± 7

-89 ± 5*

-96 ± 5*

-91 ± 5*

-91 ± 6*

-81 ± 10*

    SBT8_

-73 ± 11

-85 ± 9

-96 ± 7*

-91 ± 8*

-93 ± 8*

-90 ± 7*

-83 ± 10*

Values represent _% baseline activity; mean ± SEM; n=8 rats; all values p<0.05 compared to baseline (100%); *p<0.05 compared to NC at same Hz.

Copyright 1996 - 1998, SSAT, Inc. Revised 29 June 1998.



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