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2008 Annual Meeting Posters


Polyphenon E, An Egcg Containing Green Tea Extract That Inhibits Tumor Growth, Has No Impact On Wound Collagen Content Or Clinical Rate of Wound Infection Or Dehiscence Yet Has a Mild Inhibitory Effect On Healing As Judged By Tensometry
Aviad Hoffman*1, Raymond Baxter1, Abu Nasar1, Thomas R. Gardner3, Kumara H. Shantha1, Keith Hoffman1, Richard L. Whelan1
1Surgery, Columbia University, New York, NY; 2Pathology, Columbia University, NY, NY; 3Orhtopedics, Columbia University, NY, NY

Introduction: Catechins in green tea, in particular EGCG (epigallocatechin-3-gallate), inhibit tumor growth in many murine models. Polyphenon E (PolyE) is a concentrated mixture of catechins (65% EGCG) with minimal side affects in humans that has stronger anti-cancer effects than tea alone. Surgery is associated with increased rates of tumor growth and metastases postoperatively(postop) in the murine setting. Perioperative (periop) administration of Poly E may limit surgery’s tumor stimulatory effects. Prior to a Phase 1 study, PolyE’s effects on wound healing must be studied. This study's purpose was to assess PolyE’s impact on laparotomy wound strength and collagen content.
Methods: Balb/C mice were randomized to plain water (Control, n=20) or a 1% PolyE solution (n=25) for drinking;10 days later all mice underwent sham laparotomy that was suture closed. Mice were sacrificed on postop days (PODs) 7 or 21 and the abdominal wall pelts were harvested. The peak force and total energy required to rupture each wound was determined via tensometry; wound collagen content was determined via a Sircol Collagen assay. Serum EGCG levels were determined in 3 PolyE mice on POD1, 7, and 21 to verify drug ingestion. Results are presented as mean ± SD; data was assessed using the student’s t-test and Mann-Whitney U-test (significance, p<0.05).
Results: No wound infections or dehiscences were noted in either group. The mean peak rupture force for each group was statistically similar on POD 7 and 21. There was no statistical difference in the total energy required for rupture on POD 7, however, on POD 21 the total energy required in the PolyE group (4.1 ± 0.78 N-mm) was significantly lower than for the control mice (5.6 ± 1.7 N-mm; p=0.026). When the 2 groups wound collagen results on POD 7 were compared there was no difference noted, the same was true for the POD 21 results. EGCG was detected in the PolyE mice serum on POD 1 and POD 7; on POD21 very low levels were detected.
Conclusions: Perioperative PolyE administration was not associated with wound complications. The peak force for rupture and the collagen content was similar at both time points for the 2 groups as was total energy for rupture on POD 7; total energy on POD 21 was less for the PolyE group. PolyE warrants further study as a perioperative anti-cancer agent in the cancer setting.


 

 
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