Abstracts
1999 Digestive Disease Week

# 2155 EFFECT OF PLATELET ACTIVATING FACTOR ANTAGONIST BN-52021 ON BACTERIAL TRANSLOCATION IN ACUTE PANCREATITIS
Lourenilson J de Souza, Sao Paulo Univ Med Sch, Sao Paulo Brazil; S Jancar, KRM Leite, S N Sampietre, R S Assis, Sao Paulo Univ, Sao Paulo Brazil; MCC Machado, Sao Paulo Univ Med Sch, Sao Paulo Brazil; JEM Cunha, San Paulo Univ Med Sch, Sao Paulo Brazil

Background/Aims: Bacterial translocation is known to occur in the early phases of acute pancreatitis (AP), being demonstrated at 6h and reaching its peak at 24 hours. On the other hand, platelet-activating factor (PAF) plays an important role in the inflammatory response, increasing vascular permeability in different organ tissues. The aim of this study was to determine the possible effect of BN-52021, a potent anti-PAF substance, on bacterial translocation in experimental AP. Methods: Twenty two Wistar male rats were submitted to AP by infusion of 2.5% sodium taurocholate in a dose of 0.1ml/100g into the pancreatic duct through a P50 catheter. The animals had been previously divided in two groups: Group I (GI) - Twelve animals pretreated with 5 mg/Kg of BN-52021 given intravenously 30 minutes before induction of AP; and Group II (GII) - Ten control animals did not receive BN-52021 pretreatment. Bacterial cultures were obtained from blood samples, pancreas, liver, mesenteric lymph nodes, peritoneal fluid and cecum content at 6h after induction of AP. Results: A significant decrease in bacterial counts was observed in liver and lymph nodes obtained from animals of GI as compared to animals of GII (p<0,05). Conclusion: PAF antagonist BN-52021 reduces bacterial translocation to distant sites other than pancreas, preventing bacterial dissemination that occurs in the early phase of AP and may have beneficial effect on the evolution of this disease.

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