1999 Abstract: 2151 NEUTROPHILS ARE NOT RESPONSIBLE FOR HEPATIC INJURY IN SEVERE ACUTE PANCREATITIS
Abstracts
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The activated neutrophils have been reported to play important roles in development of the hepatic injuries associated with ischemia/reperfusion or endotoxemia. However, their involvement in hepatic injury with severe acute pancreatitis has not been confirmed. The aim of this investigation was to examine the role of neutrophils in the development of hepatic failure in acute pancreatitis. Necrotizing pancreatitis was developed on male Wistar rats by retrograde injection of 0.1 ml of 20% sodium deoxycholate into biliopancreatic ducts. Serum sample, pancreas and liver were taken 5 h after induction of pancreatitis. Serum ALT was significantly elevated in the rats with pancreatitis (172±31 IU/L) compared with in the rats with sham operation (41±5 IU/L). Myeloperoxidase activity in the liver was increased in the rats with pancreatitis (0.051±0.012 U/g tissue) compared with in the rats with sham operation (0.015±0.006 U/g tissue)(pŁ0.05), suggesting accumulation of neutrophils in the liver. However, histochemical analysis for detecting neutrophils using naphthol AS-D chloroacetate esterase cytochemical staining kit revealed that the neutrophils in the liver with pancreatitis stayed only within the vasculature, and did not transmigrate into the parenchyma unlikely to hepatic injury with endotoxemia. In order to determine significance of neutrophils in hepatic injury, anti-neutrophil monoclonal antibody, RP-3 was administrated intraperitoneally 6 h before induction of pancreatitis. With the pretreatment by RP-3, the circulating neutrophils were depleted to less than 100/mm3 from 6 to 11 h after administration. However, serum ALT was not altered with neutrophil depletion (189±22 IU/L), indicating that hepatocellular injury was not ameliorated at all with neutrophil depletion. In conclusion, although neutrophils are concentrated in the hepatic vasculature, they did not transmigrate into hepatic parenchyma. These results suggested that neutrophils are not responsible for hepatic injury in the early stage of severe acute pancreatitis. Copyright 1996 - 1999, SSAT, Inc. |