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1999 Abstract: 2127 EFFECTS OF A POTENT INHIBITOR OF FREE OXYGEN RADICALS PRODUCTION DURING LIVER NORMOTHERMIC ISCHEMIA/REPERFUSION PROCESS. EXPERIMENTAL STUDY

Abstracts
1999 Digestive Disease Week

# 2127 EFFECTS OF A POTENT INHIBITOR OF FREE OXYGEN RADICALS PRODUCTION DURING LIVER NORMOTHERMIC ISCHEMIA/REPERFUSION PROCESS. EXPERIMENTAL STUDY
Jose Eduardo M Cunha, P De Luca Neto, Emilio E Abdo, MCC Machado, F A De Luca, Mohamed I A Taha, AMM Coelho, R L Assad, Sao Paulo Univ Med Sch, Sao Paulo Brazil

Background: N2-Mercaptopropyonilglycine (N2-MPG) is a known powerful superoxide synthesis inhibitor that plays an important role in the inhibition of xantine-oxidase production. The aim of this investigation was to determine the possible beneficial effects of the antioxidative properties of N2-MPG in the prevention of metabolic and structural hepatic lesions in the normothermic ischemia/reperfusion process. Methods: Twenty-two Wistar rats were divided in two experimental study groups: Group I (n = 12) treated with N2-MPG (100mg/Kg) intravenously and Group II (n = 10) that received normal saline by the same route. Twenty-two dogs were also divided in two groups: Group III (n = 12) and Group IV (n =10) that received, respectively, the same treatment of rats of groups I and II. Ten minutes after the saline or drug injections the left hepatic lobe of the animals was submitted to 30 minutes of complete ischemia with subsequent immediate reperfusion. Blood samples were collected from all animals for determination of hepatic enzymes (ALT and AST) at 24 hours after reperfusion. Specimens of the ischemic liver lobe were obtained for histopathologic studies. Results: Average serum levels of AST and ALT in animals of Group I were, respectively, 271.00±182.46 and 261.83±161.20 and were significantly less than values observed in the control animals (Group II) (AST 2144.50±966.74) and (ALT 1869.50±1040.28) (p<0.01). The same results were observed in the dog experiments: In Group III AST (101.42±45.03) and ALT (123.25±89.99) values were significantly less altered when compared to the control Group IV: AST (182.10±76.51) and ALT (277.80±219±99) (p<0.05). Liver histopathology studies in animals of groups I and III showed mild necrosis in eight animals and moderate necrosis in four, whereas in all the animals of groups II and IV extensive parenchymal necrosis was observed. Conclusion: The antioxidant N2-MPG exerts a significant protective effect on the hepatic parenchyma when the liver is submitted to the normothermic ischemia/reperfusion process.

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