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AUTOLOGOUS MESENCHYMAL CELLS TRANSPLANTATION IN THE TREATMENT OF PERIANAL FISTULA
Pierpaolo Sileri*1, Mostafa Shalaby1,2, Fabio Nicoli1, Marco Gallinella Muzi1
1University of Rome Tor Vergata, Rome, Italy; 2Derpartment of General Surgery , Mansoura University , Mansoura, Egypt

Small 5mm sub-umbilical incision
Infiltration and Liposuction
A mixture of the solution: 250 ml physiological saline, 50 ml 2% lidocaine, 1ml adrenaline. Using ordinary 50 ml syringe connected to 20 G cannula, directed in subcutaneous tissue, in a fan-shaped manner.
After 8-10 minutes; from the same subcutaneous tissue plane, in a fan-shaped manner, and using a special 50 ml syringe connected to 13 G cannula.
Processing of Mesenchymal cells
Using a lipo-aspiration, processing, and deployment of adipose tissue device.
Using the Fistuloscope, tracing the track till the internal opening.
Advancement flap covering the internal opening
Infiltration of the mesenchymal cells around the external opening


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