Natural Orifice Transluminal Endoscopic Surgery (NOTES) may supplement the oncological providence of intraluminal resectional techniques (eg Endoscopic Submucosal Dissection) for early stage colonic neoplasia by providing nodal staging information in a truly minimally invasive fashion. Catheter-based Optical Coherence Tomography deployed via NOTES peritoneoscopy may advance this by providing high resolution (x400) optical analysis of both the colon wall from outwith the intestine as well as mesenteric lymph node architecture. This facility to provide ‘virtual biopsy’ of salient tissues (including sentinel nodes) by NOTES is demonstrated in this technical video utilizing a porcine model.