Background: Natural orifice transumbilical surgery (NOTUS) is a new surgical method whereby a laparoscopic operation is performed with all incisions placed within the umbilicus leaving no visible abdominal scars. We present our initial experience with nine patients who underwent NOTUS cholecystectomy utilizing three transumbilical laparoscopic ports.Methods: Eight patients had chronic cholecystitis and two patients had symptomatic cholelithiasis. Four of ten patients had previous open abdominal surgery. All procedures were performed utilizing a flexible-tip camera, rigid grasper, scissor, and automated clips as well as articulating dissector and hook. Transabdominal subcostal suture retraction of the gallbladder was not utilized. Results: The mean operative time was 79 minutes. There were no intraoperative or postoperative complications. There was one conversion to standard four-port laparoscopic cholecystectomy due to severe chronic inflammation. Four were discharged on the same operative day and six were discharged after a 23 hour stay. There were no postoperative complications.Conclusion: Cholecystectomy performed through transumbilical laparoscopic trochars is technically feasible and safe in well selected patients. The primary advantage is the lack of visible abdominal scars. Further clinical trials comparing NOTUS cholecystectomy to conventional laparoscopic cholecystectomy are needed.