2005 Abstracts: Local and Systemic Effects of Hypertonic Solution (NacI 7with5%) in Experimental Acute Pancreatitis
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Local and Systemic Effects of Hypertonic Solution (NacI 7with5%) in Experimental Acute Pancreatitis
Marcel C. Machado, Ana Maria M. Coelho, Vera Pontieri, Sandra N. Sampietre, Nilza A. Molan, Francisco Soriano, Andre S. Matheus, Rosely A. Patzina, Jose Eduardo M. Cunha, Irineu T. Velasco, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil
LOCAL AND SYSTEMIC EFFECTS OF HYPERTONIC SOLUTION (NaCl 7.5%) IN EXPERIMENTAL ACUTE PANCREATITIS.
Severe acute pancreatitis (AP) is characterized by hemodynamic alterations and a systemic inflammatory response, leading to a high mortality rate. Treatment of hemorrhagic shock with hypertonic saline solutions significantly reduces mortality through an improvement in the hemodynamic conditions and possibly by an anti-inflammatory effect. Therefore, hypertonic solutions could be effective in AP. Methods: We used male Wistar rats. AP was induced by intraductal 2.5% taurocholate injection. The animals were divided in 4 groups: C(n=20): control (without AP), NT(n=58): AP, not treatment, NS (n=58): received 34ml/kg of normal saline solution (NaCl 0.9%) i.v., 1 hour after AP, and HTS(n=55): 4ml/Kg of hypertonic saline solution (NaCl 7.5%) i.v., 1 hour after AP. Mean arterial blood pressure (MAP) was recorded at 0 and 2,4,24, and 48 hours after AP. After induction of AP animals were sacrificed at 2,12,24, and 48 hours to determined: IL-6, IL-10, pancreatic tissue culture and histological analysis, oxidation and phosphorylation of liver mitochondria, pulmonary myeloperoxidase activity (MPO) and mortality study. Results: In the groups NS and NT was observed a significant decrease of MAP 48h after AP (NS: 91±3mmHg) and (NT:89±3mmHg) compared to baseline (C:105 ±2mmHg) and in HTS group (102 ± 2mmHg). (p<0.05). In animals of group NT, NS and HTS, serum IL-6 and IL-10 levels were significantly higher in 2h after AP when compared to the control group. However the HTS group had a significantly lower in 2 and 12 h after AP when compared to NS and NT groups (p<0.05). The differences in histologic findings between groups were not statistically significant. After 48h of AP a significant reduction in RCR was observed in animals of group NT and NS when compared to HTS and control group (p<0.05). In group HTS it was observed a decrease of MPO activity at 24h when compared to NT and NS groups (p<0.05). A significant reduction of pancreatic infection was observed in animals of group HTS (p<0.05) and a significant reduction on mortality was observed in HTS (0/14) compared to NS (6/17-35%) and C (7/20-35%). Conclusion: Administration of HTS in experimental acute pancreatitis: attenuates hemodynamic alterations, decreases inflammatory cytokines, diminishes systemic lesions, prevents pancreatic infection and reduces the moratlity rate.
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