In 1990 Malone et al. described forming a continent appendicocestomy through which the cecum could be intermittently catheterized for administration of an antegrade continence enema. This technique has been use for management of fecal incontinence and severe constipation in children and adults. The aim of this study was to study the effects on the water, electrolyte, and acid-base balances in rabbits submitted to antegrade enema with different solutions through appendicostomy. METHODS: Forty male, New Zealand rabbits were submitted to appendicostomy, and distributed in to 4 groups, according to the antegrade enema solution on the 6^th postop: group PEG polyethylene glycol with electrolytes solution (n=10); group SF isotonic saline solution (n=10); group SG glycerin solution (n=10); and group FS sodium phosphate solution (n=10). After weighed, arterial blood gas analysis, red blood count, creatinine and electrolytes, were measured at 4 times: T1 (preoperatively); T2 (6^th postop, before enema); T3 (4h after enema); and T4 (24h after T3). RESULTS: In PEG occurred Na (T3,T4>T2; P=0.000) retention after 4h, with HCO₃ retention (T4>T1,T2,T3; P=0.004) causing alcalemia, sustained for 24h (pH: T4>T1; P=0.009). In SF occurred water isotonic retention (weigh: T3,T4>T1,T2; P=0.003; hematocrit: T1>T3,T4; T2>T4; P=0.000), and hypercloremic acidosis after 4h, which has been partially compensated in 24h (pH: T4>T3; P=0.003; HCO₃: T3<T2,T4; P=0.025; Cl: T1,T3>T2,T4; P=0.000). In SG occurred hypercloremic metabolic acidosis after 4h, which was compensated in 24h (pH: T2,T3<T4; P=0.000; HCO₃: T1,T4>T2,T3; T2>T3; P=0.000; paCO2: T3<T2,T4; P=0,000; Cl: T3>T1,T2,T4; P=0.000). In FS occurred hypernatremic dehydration (weight: T2>T1,T3,T4; T4>T3; P=0.007; Na: T3>T2,T4; P=0.000), metabolic acidosis with increased anion gap in 4h (HCO₃: T2>T3; P=0.000), hypokalemia (T1,T2>T3,T4; P=0.000), hypocalcemia (T1,T4>T2,T3; P=0.000), hypomagnesemia (T1,T2>T3,T4; P=0.000), and metabolic alkalosis with partially compensated dehydration in 24h (pH: T3<T4; P=0.001; HCO₃: T1,T4>T2,T3; P=0.000; paCO₂: T3<T1,T4; P=0.004; Na: T1>T4; P=0.000). CONCLUSIONS: All solutions cause minor alterations, on water, electrolyte or acid-base balances. The most intense ones are caused by FS and SF; and the least intense ones, by PEG and SG.